Effect of body composition intake with nano-lactic acid in rats

Prebiotic effect and probiotics lactobacillus were to one of the most famous effect-efficacy of the health conditions. It is important to reduce adult diseases risk for public health based politics. Especially in East Asian people, there were increasing the health problems related to being obsess by attributed to the Westernization of daily meals. The purpose of this study was to investigate the effect of nano-lactic acid feeding as lactobacillus in rat fed a high-fat diet, to prevention of increased body fat percentage. Male Wistar rats were individually housed in cages at temperature of 23 ± 1 degree centigrade, and 40-60% humidity. After basically 2 weeks breeding on pear feeding, 20 rats were divided 4 groups each 5 rats and were fed same calorifacient pear feeding diet and water, control (group: C) as high-carb meal, Japanese style, C with high levels lactobacillus (group: CL), high-fat same calorifacient feed and water (group: F), F with high levels lactobacillus (group: FL), all feed certain time once a day. It was delivered the lactobacillus 500 billion per 1cc by oral radio sonde tube. This animal experiment was carried out in accordance with the Japanese law, which allows experiments on laboratory animals in Nippon Sport Science University accordance to the principle of laboratory animal care. After experimental feed, it was measured lipid metabolism based in lean body mass. It was showed that induced high level lactobacillus were to block the adipose tissue fat mass. There were significantly increasing the body fat percentage in F compare to C in spite of pair feeding same calorie. However there were no significant difference in body mass in between F and C. Consequently, it was recommended to preactive intake of the nano-lactobacillus because possibility of inhibit to accumulated fat mass with high fat food. However it was inactive lactic acid because of heat-treated. Therefore, it is expected the highly-concentrated lactic bacterium as same as fiber rich diet for human. Introduction Obesity has become one of the problems in the world [1-5]. According to World Health Organization, rate of obesity has more than doubled since 1980. In 2014, more than 1.9 billion adults 18 years and older were overweight, and of those over 600 million adults were obese. Ng et al. [6] was reported to population of adults with body-mass index of 25 kg/m2 or grater increased between 1980 to 2013 from 28.8% to 36.9% in men, and from 29.8% to 38.0% in women, and prevalence of overweight or obese has increased in children and adolescents both developed country and developing country [6]. Obesity is regarded to excess accumulated of adipose tissue in the body by a lack of exercise and improper eating habits. Especially abdominal fat accumulation is one of the major risk factor for the various diseases, including diabetes [7], cardiovascular disease [8], hyperlipidemia, hypertension and chronic kindly disease [9]. Especially in East Asian people, there were increasing the health problems related to being obsess by attributed to the Westernization of daily meals. Obesity is a result of imbalance between the energy consumption and energy intake, it is necessary to control body weight to consume energy to adequate levels of physical activity [10], and to reduce energy intake [11] increased to such a highfat diet. Lactic acid bacteria are widely distributed in nature from agricultural and livestock products to inside the body of human and animal. Effect of lactic acid bacteria was considered that affect to useful substance production and intestinal flora to remain the intestinal as living microbe, and bacterial components or metabolite acts to organism directly. Bacteria used as probiotics are mainly lactic acid bacteria, probiotics was defined as ‘A live microbial feed supplement which beneficially affects the host animal by improving its intestinal microbial balance’ by Fuller [12]. Probiotic was effected health condition, Isolauri et al. [13] reported, children of atopic eczema given probiotic-supplemented formulas ware occurred improvement skin condition as compared unsupplemented children, and reduction in the concentration of soluble CD4 in serum and eosinophilic protein X in urine [13]. Also, probiotics were shown to be effective in the maintenance of remission therapy targeting colonic inflammation in ulcerative coltis or pouchitis patients [14], and to be alleviated symptom of pollinosis when yogurt supplemented with a probiotic strain was given to moderate Japanese cedar pollinosis patients [15], lactic acid bacteria as probiotics was found to have immunomodulatory effects. These effect are effectiveness of probiotic strain, it has been confirmed to even effect in the heat-killed lactic acid bacteria [16]. In addition, these lactic acid bacteria are potentially to be suppressed the increase of weight gain and body fat [17]. The purpose of this study was to investigate the effect of nano-lactic acid feeding in rat fed a high-fat diet, to prevention of increased body fat percentage. Correspondence to: Takashi Sato, PhD, 4-1-1, Osakaue Hino, Tokyo 191-8510, Japan, E-mail: [email protected]